Thursday, 08 October 2015    HomeAbout UsContact Us    

You are here: Home Research

Anti-Inflammatory Effects of Pomegranate (Punica Granatum L.) Husk Ellagitannins in Caco-2 Cells, an in Vitro Model of Human Intestine
Posted by Admin, Senior Editor in Research
Authored by PubMed
Topics: Pomegranate

  Mail To Friend    Printer Friendly Bookmark and Share

Hollebeeck S, Winand J, Hérent MF, During A, Leclercq J, Larondelle Y, Schneider YJ. Anti-inflammatory effects of pomegranate (Punica granatum L.) husk ellagitannins in Caco-2 cells, an in vitro model of human intestine. 1. Food Funct. 2012 Aug 25;3(8):875-85. Epub 2012 Jun 26.
This study aimed at evaluating the anti-inflammatory properties of a pomegranate fruit husk (PomH) polyphenolic extract, rich in punicalagin, using Caco-2 cells, an in vitro model of human intestinal epithelium. Differentiated cells in bicameral inserts were pretreated or not with a PomH extract or punicalagin, as reference, at the apical side, representing the intestinal lumen. Inflammation was then induced with a cocktail of cytokines (Il-1?, TNF? and IFN?) and LPS. After 24 h incubation, 3 pro-inflammatory markers, i.e., interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1, were assayed both at their gene transcription (qRT-PCR) and secretion (ELISA) levels. As previously described, the pro-inflammatory cocktail significantly stimulated these 3 markers, at the gene transcript and secretion levels. In inflamed cells, a significant down-regulation of the transcription of the genes encoding IL-6 and MCP-1 was observed in the presence of the PomH extract or punicalagin, while IL-8 transcription was unaffected. Both treatments also decreased the amounts of the 3 proteins with dose-response effects, but only in the apical compartment. A lowered ELISA response was also observed when either IL-6, IL-8 or MCP-1 were mixed with punicalagin in a cell-free culture medium, indicating a direct molecular interaction. In conclusion, the punicalagin-rich PomH extract tested showed anti-inflammatory properties in the Caco-2 in vitro intestinal model. It acted both on the pro-inflammatory gene transcription and protein levels, the later phenomenon being possibly due to a direct molecular trapping. These data suggest that pomegranate husk could be an interesting natural source contributing to prevent intestinal chronic inflammation.

Link to this article:   Show: HTML LinkFull LinkShort Link
Share or Bookmark this page: You will need to have an account with the selected service in order to post links or bookmark this page.

Subscribe via RSS or email:
Follow us through RSS or email. Click the RSS icon to subscribe to our feed.


Related Articles:
Add a Comment
You must be registered and logged in to comment.

Visit Vaccines.Me for information and education on vaccination.

Latest Articles
Honey and Anti-Biotic Resistance: A New Approach!
On the Rejection of Hijaamah by the Physicians (Shaykh Muhammad Bin Ibraaheem Aal Al-Shaykh)
How to Limit Efffects of Bisphenol-A Upon Children
The Harmful Effects of Bisphenol-A on the Body and Its Biochemistry
Nestle Attempting to Patent Nigella Sativa ((Black Seed)
Ajwa Dates Contain Cancer-Preventing Property
Eat Less and Live Healthier and Longer?
Efficacy of a Herbal Extract Gel in the Treatment of Gingivitis: A Clinical Study
Pomegranate Materials Inhibit Flagellin Gene Expression and Flagellar-Propelled Motility of Uropathogenic Escherichia Coli Strain CFT073
Anti-Inflammatory Effects of Pomegranate (Punica Granatum L.) Husk Ellagitannins in Caco-2 Cells, an in Vitro Model of Human Intestine

No pages found.

Most Popular
Garlic, Honey and Apple Cider Vinegar: Must Have Excellent Home Remedy
How To Eat Fruit Properly
Rocket: A Spicy Salad Leaf With Potent Health Benefits
Ibn al-Qayyim: Henna Has Many Benefits from Treating Headaches to Burns
Why We Need Protein in our Diets
Five Superfoods You Should Be Eating Everyday
Deodorant And Anti-Perspirant Dangers - Do You Know What You're Putting Under Your Armpits?
The Different Kinds Of Exercises Your Body Needs
What Foods Are Good For Your Eyesight?
Talbina: Relaxation For the Heart of the Sick Person

Archives (View more)
2014 • March
2014 • February
2013 • October
2012 • October
2012 • August
2012 • January
2011 • December
2011 • November
2011 • October
2011 • September
2011 • August
2011 • July

Copyright © 2015 . All rights reserved. RSSTagsPrivacyLegal and Terms of Use Definite Article in Arabic