Antibacterial Activity of Thymoquinone, an Active Principle of Nigella Sativa and Its Potency to Prevent Bacterial Biofilm Formation


By PubMed
Posted by Admin on Wednesday, August, 15 2012 and filed under Nigella Sativa (Black Seed)
Key topics: Nigella Sativa Black Seed

Advertisement. Report if inappropriate.

Chaieb K, Kouidhi B, Jrah H, Mahdouani K, Bakhrouf A. Antibacterial activity of Thymoquinone, an active principle of nigella sativa and its potency to prevent bacterial biofilm formation. 1. BMC Complement Altern Med. 2011 Apr 13;11:29.

BACKGROUND: Thymoquinone is an active principle of nigella sativa seed known as "Habbah Al-Sauda" in Arabic countries and "Sinouj" in Tunisia. Bacterial biofilms tend to exhibit significant tolerance to antimicrobials drugs during infections. METHODS: The antibacterial activity of Thymoquinone (TQ) and its biofilm inhibition potencies were investigated on 11 human pathogenic bacteria. The growth and development of the biofilm were assessed using the crystal violet (CV) and the 2, 3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) reduction assay. RESULTS: TQ exhibited a significant bactericidal activity against the majority of the tested bacteria (MICs values ranged from 8 to 32 ?g/ml) especially Gram positive cocci (Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis CIP 106510). Crystal violet assay demonstrated that the minimum biofilm inhibition concentration (BIC50) was reached with 22 and 60 ?g/ml for Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis CIP 106510 respectively. In addition our data revealed that cells oxidative activity was influenced by TQ supplementation. In the same way, TQ prevented cell adhesion to glass slides surface. CONCLUSION: The ability of TQ to prevent biofilm formation warrants further investigation to explore its use as bioactive substances with antibiofilm potential.

Advertisement. Report if inappropriate.
Return to Home

Health, fitness and longevity
 Based upon the principles of health
 in the Qur'an and Prophetic Traditions.
HealthyMuslim.Com

There are two bounties in which
most people lose out: good health
and free time. Al-Bukhari.
The information on this site is provided for educational purposes only. It is not intended as a substitute for professional advice of any kind.