Thursday, 18 October 2018    HomeAbout UsContact Us    

You are here: Home Research Ashwagandha

Protective Role of Ashwagandha Leaf Extract and Its Component Withanone on Scopolamine-Induced Changes in the Brain and Brain-Derived Cells
Posted by Admin, Senior Editor in Ashwagandha
Authored by PubMed
Topics: Ashwagandha Withania Somnifera

  Mail To Friend    Printer Friendly Bookmark and Share

Konar A, Shah N, Singh R, Saxena N, Kaul SC, Wadhwa R, Thakur MK. Protective role of ashwaganDHA leaf extract and its component withanone on scopolamine-induced changes in the brain and brain-derived cells. 1. PLoS One. 2011;6(11):e27265. Epub 2011 Nov 11.
BACKGROUND: Scopolamine is a well-known cholinergic antagonist that causes amnesia in human and animal models. Scopolamine-induced amnesia in rodent models has been widely used to understand the molecular, biochemical, behavioral changes, and to delineate therapeutic targets of memory impairment. Although this has been linked to the decrease in central cholinergic neuronal activity following the blockade of muscarinic receptors, the underlying molecular and cellular mechanism(s) particularly the effect on neuroplasticity remains elusive. In the present study, we have investigated (i) the effects of scopolamine on the molecules involved in neuronal and glial plasticity both in vivo and in vitro and (ii) their recovery by alcoholic extract of ashwaganDHA leaves (i-Extract). METHODOLOGY/PRINCIPAL FINDINGS: As a drug model, scopolamine hydrobromide was administered intraperitoneally to mice and its effect on the brain function was determined by molecular analyses. The results showed that the scopolamine caused downregulation of the expression of BDNF and GFAP in dose and time dependent manner, and these effects were markedly attenuated in response to i-Extract treatment. Similar to our observations in animal model system, we found that the scopolamine induced cytotoxicity in IMR32 neuronal and C6 glioma cells. It was associated with downregulation of neuronal cell markers NF-H, MAP2, PSD-95, GAP-43 and glial cell marker GFAP and with upregulation of DNA damage--?H2AX and oxidative stress--ROS markers. Furthermore, these molecules showed recovery when cells were treated with i-Extract or its purified component, withanone. CONCLUSION: Our study suggested that besides cholinergic blockade, scopolamine-induced memory loss may be associated with oxidative stress and ashwaganDHA i-Extract, and withanone may serve as potential preventive and therapeutic agents for neurodegenerative disorders and hence warrant further molecular analyses.

Link to this article:   Show: HTML LinkFull LinkShort Link
Share or Bookmark this page: You will need to have an account with the selected service in order to post links or bookmark this page.

Subscribe via RSS or email:
Follow us through RSS or email. Click the RSS icon to subscribe to our feed.


Related Articles:
Add a Comment
You must be registered and logged in to comment.

Visit Vaccines.Me for information and education on vaccination.

Latest Articles
Some Notes of Advice on Health, Disease and 'Medicine'
Cooking With Vegetable Oils Releases Toxic Cancer-Causing Chemicals, Say Experts
Cancer Simplified: Part 5 - The Initiators and Promoters of Cancer
Cancer Simplified: Part 4 - The Immune System's In-Built Anti-Cancer Mechanism
Cancer Simplified: Part 3 - Cancer Is Simply a Failure of the Immune System
Cancer Simplified: Part 2 - An Analogy for Different Perceptions About Treatment of Cancer
Cancer Simplified: Part 1 - What Is Cancer and How Does It Develop?
Honey and Anti-Biotic Resistance: A New Approach!
On the Rejection of Hijaamah by the Physicians (Shaykh Muhammad Bin Ibraaheem Aal Al-Shaykh)
How to Limit Efffects of Bisphenol-A Upon Children

No pages found.

Most Popular
Garlic, Honey and Apple Cider Vinegar: Must Have Excellent Home Remedy
How To Eat Fruit Properly
Rocket: A Spicy Salad Leaf With Potent Health Benefits
Ibn al-Qayyim: Henna Has Many Benefits from Treating Headaches to Burns
Talbina: Relaxation For the Heart of the Sick Person
Why We Need Protein in our Diets
Five Superfoods You Should Be Eating Everyday
Deodorant And Anti-Perspirant Dangers - Do You Know What You're Putting Under Your Armpits?
The Different Kinds Of Exercises Your Body Needs
What Foods Are Good For Your Eyesight?

Archives (View more)
2016 • January
2015 • November
2014 • March
2014 • February
2013 • October
2012 • October
2012 • August
2012 • January
2011 • December
2011 • November
2011 • October
2011 • September

Copyright © 2018 . All rights reserved. RSSTagsPrivacyLegal and Terms of Use Learn Quranic Arabic Online