Thursday, 21 June 2018    HomeAbout UsContact Us    









You are here: Home Research Ashwagandha


Selective Killing of Cancer Cells by Ashwagandha Leaf Extract and Its Component Withanone Involves ROS Signaling
Posted by Admin, Senior Editor in Ashwagandha
Authored by PubMed
Topics: Ashwagandha Withania Somnifera

  Mail To Friend    Printer Friendly Bookmark and Share

Ashwagandha is an exotic Indian herb which has remarkable stress-relieving properties. In addition to its excellent protective effects on the nervous system, ashwaganDHA may be a promising alternative treatment for a variety of degenerative diseases such as Alzheimer's and Parkinson's. AshwaganDHA has powerful antioxidant properties that seek and destroy the free radicals that have been implicated in aging and numerous disease states. Even more remarkable, emerging evidence suggests that ashwaganDHA has anti-cancer benefits as well.

Widodo N, Priyandoko D, Shah N, Wadhwa R, Kaul SC. Selective killing of cancer cells by ashwaganDHA leaf extract and its component Withanone involves ROS signaling. 1. PLoS One. 2010 Oct 21;5(10):e13536.
BACKGROUND AND PURPOSE: ashwaganDHA is a popular Ayurvedic herb used in Indian traditional home medicine. It has been assigned a variety of health-promoting effects of which the mechanisms remain unknown. We previously reported the selective killing of cancer cells by leaf extract of ashwaganDHA (i-Extract) and its purified component Withanone. In the present study, we investigated its mechanism by loss-of-function screening (abrogation of i-Extract induced cancer cell killing) of the cellular targets and gene pathways. METHODOLOGY/PRINCIPAL FINDINGS: Randomized ribozyme library was introduced into cancer cells prior to the treatment with i-Extract. Ribozymes were recovered from cells that survived the i-Extract treatment. Gene targets of the selected ribozymes (as predicted by database search) were analyzed by bioinformatics and pathway analyses. The targets were validated for their role in i-Extract induced selective killing of cancer cells by biochemical and molecular assays. Fifteen gene-targets were identified and were investigated for their role in specific cancer cell killing activity of i-Extract and its two major components (Withaferin A and Withanone) by undertaking the shRNA-mediated gene silencing approach. Bioinformatics on the selected gene-targets revealed the involvement of p53, apoptosis and insulin/IGF signaling pathways linked to the ROS signaling. We examined the involvement of ROS-signaling components (ROS levels, DNA damage, mitochondrial structure and membrane potential) and demonstrate that the selective killing of cancer cells is mediated by induction of oxidative stress. CONCLUSION: ashwaganDHA leaf extract and Withanone cause selective killing of cancer cells by induction of ROS-signaling and hence are potential reagents that could be recruited for ROS-mediated cancer chemotherapy.


Link to this article:   Show: HTML LinkFull LinkShort Link
Share or Bookmark this page: You will need to have an account with the selected service in order to post links or bookmark this page.

                 
  
Subscribe via RSS or email:
Follow us through RSS or email. Click the RSS icon to subscribe to our feed.

     
  

Related Articles:
Add a Comment
You must be registered and logged in to comment.





Visit Vaccines.Me for information and education on vaccination.


Latest Articles
Some Notes of Advice on Health, Disease and 'Medicine'
Cooking With Vegetable Oils Releases Toxic Cancer-Causing Chemicals, Say Experts
Cancer Simplified: Part 5 - The Initiators and Promoters of Cancer
Cancer Simplified: Part 4 - The Immune System's In-Built Anti-Cancer Mechanism
Cancer Simplified: Part 3 - Cancer Is Simply a Failure of the Immune System
Cancer Simplified: Part 2 - An Analogy for Different Perceptions About Treatment of Cancer
Cancer Simplified: Part 1 - What Is Cancer and How Does It Develop?
Honey and Anti-Biotic Resistance: A New Approach!
On the Rejection of Hijaamah by the Physicians (Shaykh Muhammad Bin Ibraaheem Aal Al-Shaykh)
How to Limit Efffects of Bisphenol-A Upon Children

Pages
No pages found.

Most Popular
Garlic, Honey and Apple Cider Vinegar: Must Have Excellent Home Remedy
How To Eat Fruit Properly
Rocket: A Spicy Salad Leaf With Potent Health Benefits
Ibn al-Qayyim: Henna Has Many Benefits from Treating Headaches to Burns
Why We Need Protein in our Diets
Talbina: Relaxation For the Heart of the Sick Person
Five Superfoods You Should Be Eating Everyday
Deodorant And Anti-Perspirant Dangers - Do You Know What You're Putting Under Your Armpits?
The Different Kinds Of Exercises Your Body Needs
What Foods Are Good For Your Eyesight?

Archives (View more)
2016 • January
2015 • November
2014 • March
2014 • February
2013 • October
2012 • October
2012 • August
2012 • January
2011 • December
2011 • November
2011 • October
2011 • September


Copyright © 2018 . All rights reserved. RSSTagsPrivacyLegal and Terms of Use Learnarabic.Com